The aim of the project is to describe the radial distribution of deuterium in Scots pine tree rings in order to develop a sampling methodology in 2H2-labeled water experiments . The experiment is located in Rosinedal experimental forest where 2H2-labeled water was added to soil. Tree ring samples were collected from two trees close to the labeled area in 2019. The sampling was carried out in four different heights (ground level, 1.3m, 2.0m and from the beginning of the living crown). From each height, 16 core samples were taken around the stem. In addition, core samples were taken from coarse roots.